Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.105575 |
Chromosome: | chromosome 13 |
Location: | 1541606 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g572900 | THO3 | (1 of 1) 3.4.24.70 - Oligopeptidase A; Oligoendopeptidase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACCTCCCCCCCGGTTTAGTTTGGGCTG |
Internal bar code: | GTGAGCTCAAAACCCGCAGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 423 |
LEAP-Seq percent confirming: | 98.8402 |
LEAP-Seq n confirming: | 767 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGGGAAGTAGGAGGGGAAG |
Suggested primer 2: | CAGCCCGATTCACTATCCAT |