Insertion junction: LMJ.RY0402.105575_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.105575
Chromosome:chromosome 13
Location:1541606
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g572900 THO3 (1 of 1) 3.4.24.70 - Oligopeptidase A; Oligoendopeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACCTCCCCCCCGGTTTAGTTTGGGCTG
Internal bar code:GTGAGCTCAAAACCCGCAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:423
LEAP-Seq percent confirming:98.8402
LEAP-Seq n confirming:767
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGGAAGTAGGAGGGGAAG
Suggested primer 2:CAGCCCGATTCACTATCCAT