| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.105576 |
| Chromosome: | chromosome 16 |
| Location: | 1420177 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652300 | CMA2,TTL13 | Chlamydomonas-specific membrane protein family A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTGTCCCGTGCGGCCGTGCCGGTGTGG |
| Internal bar code: | TACCCGACTCTGGCTTGCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1184 |
| LEAP-Seq percent confirming: | 99.3548 |
| LEAP-Seq n confirming: | 462 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGACCGTGACACACAGGA |
| Suggested primer 2: | AGGCACGGAAGTACGGTATG |