| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105577 |
| Chromosome: | chromosome 3 |
| Location: | 468419 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145107 | (1 of 3) K10406 - kinesin family member C2/C3 (KIFC2_3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGCTGCTTGCGTTATAAGTCGTTGTCT |
| Internal bar code: | CGGTCTTGCCTGTGAGCGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 99.6719 |
| LEAP-Seq n confirming: | 1215 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCCAATATCCTTGCTCA |
| Suggested primer 2: | AGCTGAAGCAGAAGCAGGTC |