| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105617 |
| Chromosome: | chromosome 6 |
| Location: | 3620648 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278125 | (1 of 1) PF09379 - FERM N-terminal domain (FERM_N) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGGGGCAGTCGGCGGGCGCGCGGTGCA |
| Internal bar code: | GGCCTCTGCAAAGCCCTGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 98.7814 |
| LEAP-Seq n confirming: | 1297 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGTCTGCAGGCTGCTCT |
| Suggested primer 2: | ACATTTCTGCCAGTCCCAAC |