| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105638 |
| Chromosome: | chromosome_10 |
| Location: | 2920863 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g440250 | PGM19 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTCAGTGAAGCACATGCGCCCAGTCTTCT |
| Internal bar code: | GGTTACCGCGGGTTCTACCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1318 |
| LEAP-Seq percent confirming: | 99.5243 |
| LEAP-Seq n confirming: | 5440 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACTTGCCTCAGTTCTTCG |
| Suggested primer 2: | GACGATGTTCGAGTTGCTGA |