| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.105676 |
| Chromosome: | chromosome 12 |
| Location: | 7646025 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554650 | REC8 | cohesin complex subunit, putative meiotic isoform; (1 of 3) PF04824 - Conserved region of Rad21 / Rec8 like protein (Rad21_Rec8) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGAGATAATTGAGGCACGCCCGCGACG |
| Internal bar code: | GTCGACGTGAAAGGGAGCGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 571 |
| LEAP-Seq percent confirming: | 98.8862 |
| LEAP-Seq n confirming: | 2042 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACAAGCTTCCCTTTGCT |
| Suggested primer 2: | CGTCCTTCTCGCTATCCAAG |