| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.105681 |
| Chromosome: | chromosome 1 |
| Location: | 2789961 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016542 | (1 of 1) IPR000104//IPR029063 - Antifreeze protein, type I // S-adenosyl-L-methionine-dependent methyltransferase | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAGCCAGCGGACGGCGAGGAGCCGGCA |
| Internal bar code: | ACTAATGGACCGCATATGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 434 |
| LEAP-Seq percent confirming: | 99.691 |
| LEAP-Seq n confirming: | 7742 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACGGAGAAGATGGATCA |
| Suggested primer 2: | CTGCAATATTTGCGGTCCTT |