Insertion junction: LMJ.RY0402.105686_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.105686
Chromosome:chromosome 10
Location:979628
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g424775 CTP2,PCH1,PAA1 Copper transporting ATPase and Cu chaperone for CTP1; (1 of 1) PTHR24093:SF124 - COPPER-TRANSPORTING ATPASE PAA1, CHLOROPLASTIC 3'UTR|intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAACGCTGCTGTGATGCACGGAACCATC
Internal bar code:CGTTGCATGGGGGGGAAGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:428
LEAP-Seq percent confirming:98.8372
LEAP-Seq n confirming:4845
LEAP-Seq n nonconfirming:57
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTCGTGAGGTTCCCTT
Suggested primer 2:ACACACATACACACGCACCC