| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.105695 |
| Chromosome: | chromosome 3 |
| Location: | 4873782 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179300 | SRH6 | (1 of 1) K11367 - chromodomain-helicase-DNA-binding protein 1 [EC:3.6.4.12] (CHD1); SNF2-related DNA/RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCACACAGGGGCTTTTCCATTGGCCCCG |
| Internal bar code: | CGGGTTCATGCTTGAGGATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 752 |
| LEAP-Seq percent confirming: | 93.4641 |
| LEAP-Seq n confirming: | 286 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATATGCAATGCCGCTACC |
| Suggested primer 2: | ACGAAAGGCAAGCAAGTTGT |