| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.105696 |
| Chromosome: | chromosome 12 |
| Location: | 94791 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483950 | MDN4,MDH4 | (1 of 3) K00026 - malate dehydrogenase (MDH2); Malate dehydrogenase 4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACAATGACAAGGTAGCCCACCCAAGGT |
| Internal bar code: | GGGATAGATAATAGGTGAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 714 |
| LEAP-Seq percent confirming: | 98.6891 |
| LEAP-Seq n confirming: | 2409 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATAAGCTGCTTGGGGCTG |
| Suggested primer 2: | GAGGGTTACGCACGTCATTT |