| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.105714 |
| Chromosome: | chromosome 1 |
| Location: | 6477431 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g046052 | (1 of 6) 2.7.11.21 - Polo kinase / Polo-like kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAATCATCAAGCCAAATAACCATCAAGGT |
| Internal bar code: | GTACGATGTTAGAATCAATGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1054 |
| LEAP-Seq percent confirming: | 99.6766 |
| LEAP-Seq n confirming: | 4007 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGCTTGCCTAAAAGCAC |
| Suggested primer 2: | GTGTCGTACGGTAGGGCAGT |