| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105733 |
| Chromosome: | chromosome 6 |
| Location: | 7690936 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g301806 | (1 of 71) PF12499 - Pherophorin (DUF3707) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACGAGTGGATCTCGGTTCTGTTGCAGC |
| Internal bar code: | GCAATATTGTCCTGGCGTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 262 |
| LEAP-Seq percent confirming: | 99.6696 |
| LEAP-Seq n confirming: | 905 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGCTGTGTCTCAAATCA |
| Suggested primer 2: | AGCGCAGTGAAGAAGGTCAT |