| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105822 |
| Chromosome: | chromosome 10 |
| Location: | 967062 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424750 | PPD1 | (1 of 2) 2.7.9.1 - Pyruvate, phosphate dikinase / Pyruvate,phosphate dikinase; Pyruvate phosphate dikinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGCATCTGCATCGTGCAGTTGTGTGGT |
| Internal bar code: | CACGCGTCAATGAAGTGACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 99.7986 |
| LEAP-Seq n confirming: | 991 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATTGCCGTGTGCAGTAGG |
| Suggested primer 2: | CCGTGACTGAGGTAGGTGGT |