| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.105824 |
| Chromosome: | chromosome_1 |
| Location: | 1824669 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g009800 | FAP289 | Flagellar Associated Protein, coiled-coil | sense | 5'UTR_intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTGAAAGTACCTCATAACGGGTTTGCATT |
| Internal bar code: | GGCGGGAGTCCCCTGGATGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 83.6409 |
| LEAP-Seq n confirming: | 997 |
| LEAP-Seq n nonconfirming: | 195 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACAACTCTTCTTCCAGCG |
| Suggested primer 2: | AACCGGGAGAAGGAAGGTTA |