| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105849 |
| Chromosome: | chromosome_2 |
| Location: | 7353831 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g145100 | FAP39 | P-type ATPase/cation transporter, flagellar associated | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGCGCTTACGTGCGCTGTCACGTTTACC |
| Internal bar code: | CCGAAAACCTCTCTCAGGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 966 |
| LEAP-Seq percent confirming: | 99.5509 |
| LEAP-Seq n confirming: | 9311 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAACATGGACAGGAACG |
| Suggested primer 2: | GTAAGAGCCTGCCTTCATGC |