| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105849 |
| Chromosome: | chromosome_2 |
| Location: | 7353841 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g145100 | FAP39 | P-type ATPase/cation transporter, flagellar associated | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGTGTGTGCCCGACTCGCGACCTTGCGCGC |
| Internal bar code: | GAAGTTTATGTATTTTCATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 99.2443 |
| LEAP-Seq n confirming: | 1576 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAACATGGACAGGAACG |
| Suggested primer 2: | GTAAGAGCCTGCCTTCATGC |