Insertion junction: LMJ.RY0402.105900_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.105900
Chromosome:chromosome 4
Location:3412599
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g227550 (1 of 39) IPR001932 - PPM-type phosphatase domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACGGTGCTGCTCTGCTGCGAGCGGTTGC
Internal bar code:GCATCGGCAATATGGATCATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:116
LEAP-Seq percent confirming:98.818
LEAP-Seq n confirming:418
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTCGCTGTTGCTGTTAC
Suggested primer 2:AGCCTCTTTGATGGTGATGG