Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.105904 |
Chromosome: | chromosome_12 |
Location: | 7085134 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g559400 | PDE12 | 3\',5\'-cyclic-nucleotide phosphodiesterase | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTAGTGGTGCTCCTCTGCCACCTCAACCGA |
Internal bar code: | AGCCCCGCTCGCGAATATCGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 277 |
LEAP-Seq percent confirming: | 99.6652 |
LEAP-Seq n confirming: | 1786 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTGGGTTTCCTGTCATTCT |
Suggested primer 2: | TGTGACTTGGTGGGTTTCAA |