| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105912 |
| Chromosome: | chromosome 3 |
| Location: | 8147682 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200600 | KIN14A1,KIN14A-1,KIN14-4 | Kinesin motor protein; (1 of 2) K10405 - kinesin family member C1 (KIFC1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTCCCAGGCGCGCAAGCTGGAGGAGCA |
| Internal bar code: | CACGATGGTGTGTTTTGGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 571 |
| LEAP-Seq percent confirming: | 97.6923 |
| LEAP-Seq n confirming: | 508 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTGTGGGTCTTGGTGTG |
| Suggested primer 2: | CCGCTGATGTAGAGCATGAA |