| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105913 |
| Chromosome: | chromosome 9 |
| Location: | 7188286 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412175 | (1 of 2) 3.7.1.8 - 2,6-dioxo-6-phenylhexa-3-enoate hydrolase / HOHPDA hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGCTCCAGCTACGCGGCCCTGAACGCC |
| Internal bar code: | GTGCTAGATTAGCTGAATTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 98.4615 |
| LEAP-Seq n confirming: | 256 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACCGTACCACAGAAGCCC |
| Suggested primer 2: | ACGGTGTCGTTTTACTTGCC |