| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.105923 |
| Chromosome: | chromosome 3 |
| Location: | 4769015 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178200 | CCT10 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACACAGGCTCAAGGCCCTCCAAGCGGCG |
| Internal bar code: | TATATGTGTGGGGATGGGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 98.3539 |
| LEAP-Seq n confirming: | 2151 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGAACCAAACGAGCTTTC |
| Suggested primer 2: | CATACCGTACTTGGAGGCGT |