Insertion junction: LMJ.RY0402.105926_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.105926
Chromosome:scaffold 36
Location:5439
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre36.g759597 (1 of 2) IPR001841//IPR013083//IPR024766 - Zinc finger, RING-type // Zinc finger, RING/FYVE/PHD-type // Zinc finger, RING-H2-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGTGGCCACCCCACCCGATACCACGTC
Internal bar code:GAAGTCCCCGCTGGGGTAGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:92
LEAP-Seq percent confirming:96.3964
LEAP-Seq n confirming:107
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGTACCCTCATTGTCTGC
Suggested primer 2:ACACACACACACACCACACG