| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105934 |
| Chromosome: | chromosome 10 |
| Location: | 4336561 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451100 | (1 of 1) PTHR11005//PTHR11005:SF2 - LYSOSOMAL ACID LIPASE-RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGAAGCTAGAATGAACGTAGGCCTGCT |
| Internal bar code: | GCGTTTCATCATGGTCCGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 99.5441 |
| LEAP-Seq n confirming: | 1310 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGTGTTGGTAAGCCGCAA |
| Suggested primer 2: | ATGTATACTCGTGGGGCTGC |