Insertion junction: LMJ.RY0402.105938_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.105938
Chromosome:chromosome 2
Location:4857661
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g103250 MRP5 (1 of 3) PTHR24223//PTHR24223:SF252 - FAMILY NOT NAMED // PROTEIN MRP-2; ABC transporter, multidrug resistance associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCTTCGACTCCCACTCCGACCCCGAGC
Internal bar code:CGACATCCGCAGGACTGTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:44
LEAP-Seq percent confirming:97.3085
LEAP-Seq n confirming:470
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAGAGCTCCCTCATTGTC
Suggested primer 2:AGCAGTACTAGGTCGGCGTC