| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.105945 |
| Chromosome: | chromosome 14 |
| Location: | 3935253 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632950 | NOT1 | (1 of 1) K12604 - CCR4-NOT transcription complex subunit 1 (CNOT1, NOT1); Component of CCR4-NOT transcriptional regulator complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGCGGCAGGTGCGGGCGGAAGCAAACA |
| Internal bar code: | GGACCGTTCCGCTCATATCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 813 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 189 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGGTAATGCGGGTTGAG |
| Suggested primer 2: | GTCTCCGAGAATGGTGGTGT |