| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.105946 |
| Chromosome: | chromosome 17 |
| Location: | 3701569 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726983 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATTTACATTGTGGCAAAGGCTGTCACAT |
| Internal bar code: | ATACTCAGAGGTCCTAAATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 99.5921 |
| LEAP-Seq n confirming: | 1709 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAACGTCAAGGCAGATA |
| Suggested primer 2: | TACGGGCCCATAACTAGCAC |