Insertion junction: LMJ.RY0402.105962_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.105962
Chromosome:chromosome 6
Location:5160696
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g281450 SRR22 Scavenger receptor cysteine rich (SRCR) protein; (1 of 1) 1.4.3.13//2.7.11.1 - Protein-lysine 6-oxidase / Lysyl oxidase // Non-specific serine/threonine protein kinase / Threonine-specific protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAACAGTAACAGCAGCGACGAGAGCGAGC
Internal bar code:CAATTGGGGCGTGATGCCCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:682
LEAP-Seq percent confirming:99.5957
LEAP-Seq n confirming:739
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACGAGTATGAACAGGGACGG
Suggested primer 2:CACATGCATAACCTATGGCG