| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105970 |
| Chromosome: | chromosome 3 |
| Location: | 9042343 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g210513 | (1 of 2) K05894 - 12-oxophytodienoic acid reductase (OPR) | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGATGAAGCAAAAGTATGCGGCTAAAC |
| Internal bar code: | CGTATGGCAAGAGTCGTAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 99.0512 |
| LEAP-Seq n confirming: | 1566 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCTGTTCCTGATCGTTTG |
| Suggested primer 2: | GACGTTTCACTGACGAGGGT |