| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.106008 |
| Chromosome: | scaffold 26 |
| Location: | 62129 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre26.g756997 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGGTGAAAATGTTGGGTTGATGTGGTA |
| Internal bar code: | ACGCAACGAATAGGCGGTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 82.545 |
| LEAP-Seq n confirming: | 1972 |
| LEAP-Seq n nonconfirming: | 417 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCACCTACTCCATGTCC |
| Suggested primer 2: | CAGTAGCGGCGGCAGTAG |