| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106012 |
| Chromosome: | chromosome 7 |
| Location: | 2426247 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328850 | CNK8 | NimA-related protein kinase; (1 of 1) PTHR24361:SF240 - PROTEIN MTK-1, ISOFORM B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCTAAAGCTGATTGGGAAGGGCACTTA |
| Internal bar code: | GGGTTCTTTTGTGTTCAGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 716 |
| LEAP-Seq percent confirming: | 99.6181 |
| LEAP-Seq n confirming: | 3391 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTGTGCTTGAAGAACTG |
| Suggested primer 2: | TTTTGAGTGTCTCGGAGGCT |