| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.106018 |
| Chromosome: | chromosome 1 |
| Location: | 1010581 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005750 | (1 of 11) IPR002350 - Kazal domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCAAGTAAGTGCGGGGTCGCTAGTTAG |
| Internal bar code: | ACGCACTTGGGAACCTGAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 89.7143 |
| LEAP-Seq n confirming: | 314 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACACGCCATCTACCAACC |
| Suggested primer 2: | ACACACACACACACACACCG |