| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.106059 |
| Chromosome: | chromosome 14 |
| Location: | 1760980 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619800 | SPO11B,TOP4 | meoisis-related DNA topoisomerase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGGCGCATAACCGCCGCGCCCAGGTA |
| Internal bar code: | CGGTTCTGCCAGGCCGGCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 204 |
| LEAP-Seq percent confirming: | 98.6111 |
| LEAP-Seq n confirming: | 284 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACCGCCGAAGCTGAGTA |
| Suggested primer 2: | TGCTATCCATCCTCCTCACC |