Insertion junction: LMJ.RY0402.106061_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.106061
Chromosome:chromosome 12
Location:4152210
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g518150 FAL17 Expressed protein of unknown function; (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGGAAACCCCTGCACTCAGACACCTGCC
Internal bar code:TTATCAAAACTGCGCCCGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:449
LEAP-Seq percent confirming:97.8369
LEAP-Seq n confirming:2759
LEAP-Seq n nonconfirming:61
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTCCATCAAACATCACAG
Suggested primer 2:ACTTGTAACCCAATCTGCCG