Insertion junction: LMJ.RY0402.106065_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.106065
Chromosome:chromosome 9
Location:5517292
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g400516 (1 of 6) K04710 - ceramide synthetase (CERS) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACGTCCGCATGGTGAGGCACCGAGGCAG
Internal bar code:GTAGATTCACGAGGGGCCGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:655
LEAP-Seq percent confirming:99.0212
LEAP-Seq n confirming:1214
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGGTGTTTTTCCTGTCCT
Suggested primer 2:AGGTGCTGCGAACTGAGATT