Insertion junction: LMJ.RY0402.106101_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.106101
Chromosome:chromosome 8
Location:3582107
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g375800 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGGTGGTGGCTGTGGCGGCTTTGGGGAG
Internal bar code:GGTGTCCGACCGGGACGATGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:699
LEAP-Seq percent confirming:94.2774
LEAP-Seq n confirming:972
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGCTCGATAATGATGGGT
Suggested primer 2:TCGATGTTTGTGGTTCGGTA