| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.106102 |
| Chromosome: | chromosome 8 |
| Location: | 4045952 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379300 | (1 of 1) PTHR12459//PTHR12459:SF6 - UNCHARACTERIZED // TRANSMEMBRANE PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATTAGGAGCTGCCTCTGGCCTCCTGGA |
| Internal bar code: | GGACAAAAACAATCTTCCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 98.9873 |
| LEAP-Seq n confirming: | 3519 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTCGATTGAGGAGGAAC |
| Suggested primer 2: | AGTCAGTCAGTCACCCACCC |