Insertion junction: LMJ.RY0402.106104_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.106104
Chromosome:chromosome 9
Location:2420130
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g391600 MEP1 Putative IgA-specific metalloendopeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCGGCACACTGGCGGAAGCGAAGGCGGA
Internal bar code:CGTCCCAACAATTTTATCCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:282
LEAP-Seq percent confirming:85.1485
LEAP-Seq n confirming:172
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCGAACAGCGGTTACAAT
Suggested primer 2:TTCTGCCCGTTCTGTCTTTC