| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.106114 |
| Chromosome: | chromosome 6 |
| Location: | 2109811 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265250 | HTR12 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGATAATAATTCGTAGCTGGGTTGTGG |
| Internal bar code: | AGGCGATGCAACTCACGCAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 97.4222 |
| LEAP-Seq n confirming: | 1096 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTACTTTAACCGTCGCCA |
| Suggested primer 2: | TTCCCAGAACCCAGAAGATG |