| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106130 |
| Chromosome: | chromosome_14 |
| Location: | 799332 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613050 | antisense | intron|outside_mRNA | ||
| Cre14.g613075 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTGCGCACGCGCCGCATGTGTGTACTGAA |
| Internal bar code: | GGTGATTTTTAGTTAGTCACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 347 |
| LEAP-Seq percent confirming: | 77.4451 |
| LEAP-Seq n confirming: | 388 |
| LEAP-Seq n nonconfirming: | 113 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATCAATCAACTACCGCC |
| Suggested primer 2: | CTTGTAGTGGAGGAGCAGGC |