| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106137 |
| Chromosome: | chromosome 12 |
| Location: | 1273801 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489250 | (1 of 3) PTHR23213//PTHR23213:SF214 - FORMIN-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATAGACCCTGAGGATGTCCTGGAGCTG |
| Internal bar code: | GCACGGGGCCGGATCGCAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 351 |
| LEAP-Seq percent confirming: | 99.8244 |
| LEAP-Seq n confirming: | 3980 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGGTGTATGAAAGCAAAT |
| Suggested primer 2: | TGCGGCATACCGTGTAAATA |