| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106137 |
| Chromosome: | chromosome 17 |
| Location: | 5189588 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735600 | TGL21 | (1 of 3) PTHR21493//PTHR21493:SF153 - CGI-141-RELATED/LIPASE CONTAINING PROTEIN // PROTEIN T08B1.4, ISOFORM B-RELATED; Putative triacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTCGGCCCGCCGCGGAACATCTCCTTTG |
| Internal bar code: | GTGTGATAGCTCCAGTGTGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 755 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTGGGCTACTCGGATTG |
| Suggested primer 2: | ACTTTGGTCTTGTCGGTTGC |