| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.106197 |
| Chromosome: | chromosome 6 |
| Location: | 6747678 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294600 | (1 of 3) PF15227 - zinc finger of C3HC4-type, RING (zf-C3HC4_4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCGGGGTACTAGGTCGGTGGCCCTGG |
| Internal bar code: | GCAAGCGAGTCCCACCACCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1056 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAACGCCGTAAAAGGACT |
| Suggested primer 2: | AGGAGGAAGGTCTTGTGGGT |