| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.106225 |
| Chromosome: | chromosome 16 |
| Location: | 5288474 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682552 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGCCGCCGCCATCAGCGGAGCAGTATT |
| Internal bar code: | AGAATATACACGCGCGTACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 99.0138 |
| LEAP-Seq n confirming: | 1004 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAACCGTCCAGCAACAGG |
| Suggested primer 2: | TTCAGTAGCCATGACAAGCG |