| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106262 |
| Chromosome: | chromosome 10 |
| Location: | 2426208 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435750 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGTCAGTTGATTGCGCGCGCTTGCACAAT |
| Internal bar code: | TACCAGGCCCTAAATGGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 664 |
| LEAP-Seq percent confirming: | 99.8444 |
| LEAP-Seq n confirming: | 5132 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTACCCACCACCATGAAT |
| Suggested primer 2: | GTGCGTACATACATGCTGGG |