| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106270 |
| Chromosome: | chromosome 12 |
| Location: | 3984750 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516700 | 5'UTR | ||
| Cre12.g516717 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAATCCATGTGCAACGTTGTCAATTACAT |
| Internal bar code: | CGAGAAGCCGAAAACACTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1041 |
| LEAP-Seq percent confirming: | 99.2579 |
| LEAP-Seq n confirming: | 535 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCTATAATTGCGGCTGG |
| Suggested primer 2: | CTCAAGGGGGTTGTGACACT |