Insertion junction: LMJ.RY0402.106320_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.106320
Chromosome:chromosome 8
Location:722965
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g360450 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTGTCGTTACGCCATCCACAGTTCCACA
Internal bar code:GGGCGCGACCTAGGGACACATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1092
LEAP-Seq percent confirming:99.6727
LEAP-Seq n confirming:19184
LEAP-Seq n nonconfirming:63
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGACAACAAGGCAAGGAG
Suggested primer 2:CTTCTGGAGAGTGGAGGTCG