| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.106323 |
| Chromosome: | chromosome 5 |
| Location: | 2726559 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236850 | NAT14 | N-acetyltransferase; (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATACGCGTGCATTAAGCTCCATGCGACA |
| Internal bar code: | TCATCGCGATTCCAGCTTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 98.5337 |
| LEAP-Seq n confirming: | 336 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACAACCTCTACCGTAGCC |
| Suggested primer 2: | AGTGCAACATCATGCCACAT |