| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.106345 |
| Chromosome: | chromosome 9 |
| Location: | 180337 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386740 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCGACCCCCTCATTGCACTTGCAACG |
| Internal bar code: | TTCAATTCGCAAAGGGAGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 614 |
| LEAP-Seq percent confirming: | 98.9435 |
| LEAP-Seq n confirming: | 2716 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACAAATGCCCTCTTGTC |
| Suggested primer 2: | CCGAGCACTTTATGCTGTGA |