Insertion junction: LMJ.RY0402.106365_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.106365
Chromosome:chromosome 17
Location:1941097
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g710700 (1 of 4) PTHR23257:SF490 - DUAL SPECIFICITY PROTEIN KINASE SHKE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGAAGTTCTGGGCCGCCAAAGTTTGGAG
Internal bar code:GTGCGGGTTGCTGATTCTCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:503
LEAP-Seq percent confirming:97.619
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCAATCAATTCCTTCCTC
Suggested primer 2:TCGACTTCGTCAGCCTACCT