| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106374 |
| Chromosome: | chromosome 3 |
| Location: | 6457543 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194750 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCATCGCTGCACGAGGACTCGCTGCGT |
| Internal bar code: | AAGGCATGCGTCTACCGGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 97.8495 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAAGTGTCTTCAGTGCC |
| Suggested primer 2: | ACAGTCGACACCCCATAAGC |